Enhanced In Situ Aerobic Bioremediation
Enhanced in situ aerobic bioremediation is defined and practiced in many ways. Biostimulation is one method. It is accomplished by supplying the indigenous microbes with inorganic nutrients used to build cell structures. Oxygen, as an electron acceptor may be delivered to subsurface microbes. Surface active agents are sometimes used to relieve the bonds between hydrocarbons and soil particles. Bioaugmentation or adding microbes that are naturally adept in degrading the specific contaminants on site is also used to speed the bioremediation process.
The patented and proprietary technologies by CleanEARTH Solutions enhanced in situ aerobic hydrocarbon bioremediation by combining a unique union of chemistry and biology. A continuous flow of available oxygen is supplied by a highly specialized biological process in BioActivate. Hydrocarbon EM and Surface EM maintain a metabolically active population of augmented and/or indigenous microbes with organic emulsifiers and surfactants. Life and cell reproduction are both maintained as an expanding population of bacteria flow through water and porous matrices.
Microbes supporting emulsifiers and enhanced bioavailability
The intermolecular adhesions within the hydrocarbon mass are compromised. Hydrocarbons are dispersed and emulsified, upon contact, into colloidal droplets. These energy serving hydrocarbon droplets are surrounded and encapsulated by water. This microbe supporting colloidal film established upon contact is held stable (will not phase out) thereby providing a substrate and water interface, or lifeline, critical to the bioremediation process.
Highly accessible hydrocarbons
When hydrocarbons are emulsified and suspended at the particle level, they flow more easily through soil pore spaces, particle voids and interstitial spaces. The surface active agents in water improve the flow and bring the droplet size hydrocarbons through various matrices to the microbes (β). Conversely, the solutions carry the microbes, their enzymes and nutrients to the hydrocarbons (à). These two methods used to connect metabolically active microbes to the hydrocarbons increase the total volume of accessible energy.
When a microbe cannot access its energy source, it will cease activity and may die. This fact of life remains true no matter how veracious the organism. When a lion, for example, cannot access food and water the population in that region will become extinct. Food source accessibility is vital to microbial life and reproduction. Hydrocarbons that are made highly accessible and easy to utilize breed complete and expeditious biodegradation.
Microbes are exposed to a nearly infinite amount of hydrocarbon surface area once they have been emulsified and held in suspension at the particle level. Take for example a one centimeter size cube. Slice that cube into 10 nanometer size cubes and the available surface area increased by over 100,000 times. Dramatically enhanced bioavailability (living and feeding space) limits microbe crowding and competition. Toxic waste accumulation too is attenuate as byproducts are emulsified and dispersed and made available to other microbes for consumption.
Microbe supporting chemistry
The solutions by CleanEARTH Solutions redefine the definition of enhanced in situ bioremediation. The emulsifiers and surfactants are organic. A stable emulsion established upon contact is achieved by using ingredients that double as an effective energy source. Microbes can be added at the manufacturing level where they begin repairing the cell structures and enzyme systems to rapidly initiate bioremediation once applied. What’s more, a great population of microbes will remain metabolically active even when not in contact with hydrocarbons. Microbes will secrete their enzymes and reproduce to successfully overwhelm and degrade many levels of petroleum hydrocarbons (ppm).